catalog number :
MBS760052
products type :
ELISA Kit
products full name :
Human Hepatitis B Surface Antigen ELISA Kit
products short name :
[Hepatitis B Surface Antigen]
other names :
[hepatitis B surface antigen, partial; Hepatitis B surface antigen; Hepatitis B surface antigen]
products gene name :
[HbsAg]
uniprot entry name :
A3KC59_HBV
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
other info1 :
Samples: Serum or plasma. Assay Type: Sandwich
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. HBsAb was pre-coated onto 96-well plates. The test samples were added to the wells, unbound conjugates were washed away with wash buffer. Then added HRP conjugated HBsAb, if there were any HBsAg in the samples, it would form a HBsAb- HBsAg - HRP- HBsAb complex. TMB substrates were used to visualize HRP enzymatic reaction. It was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The optical density of developed color is read with a suitable photometer at 450nm with a selected reference wavelength within 650 nm.
ncbi acc num :
BAF48754.1
ncbi mol weight :
14,390 Da
size4 :
10x96-Strip-Wells