ELISA/assay

Human Total Apelin (Apelin) Elisa kit

MBS756419

48-Strip-Wells-(Competitive)

440 USD

ELISA Kit

Human Total Apelin (Apelin) Elisa kit

[Apelin]

Human

This assay has high sensitivity and excellent specificity for detection of AOAH. No significant cross-reactivity or interference between AOAH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AOAH and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This AOAH ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine AOAH. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: AOAH ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AOAH antibody and an AOAH-HRP conjugate. The assay sample and buffer are incubated together with AOAH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOAH concentration since AOAH from samples and AOAH-HRP conjugate compete for the anti-AOAH antibody binding site. Since the number of sites is limited, as more sites are occupied by AOAH from the sample, fewer sites are left to bind AOAH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOAH concentration in each sample is interpolated from this standard curve.

6708146

AAF25815.1

apelin: Endogenous ligand for APJ, an alternative coreceptor with CD4 for HIV-1 infection. Inhibits HIV-1 entry in cells coexpressing CD4 and APJ. Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Belongs to the apelin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: Xq25. Cellular Component: extracellular region; extracellular space; perinuclear region of cytoplasm. Molecular Function: apelin receptor binding; G-protein-coupled receptor binding; hormone activity; receptor binding. Biological Process: drinking behavior; immune response; lactation; negative regulation of blood pressure; negative regulation of vasoconstriction; positive regulation of adrenocorticotropic hormone secretion; positive regulation of cell proliferation; positive regulation of corticotropin-releasing hormone secretion; positive regulation of heat generation; positive regulation of phosphorylation; positive regulation of vasodilation; regulation of respiratory gaseous exchange; regulation of the force of heart contraction; signal transduction

48-Strip-Wells-(Competitive)

440 USD

48-Strip-Wells-(Sandwich)

440

96-Strip-Wells-(Competitive)

640

96-Strip-Wells-(Sandwich)

640

5x96-Strip-Wells-(Competitive)

2895

5x96-Strip-Wells-(Sandwich)

2895

10x96-Strip-Wells-(Competitive)

5415

10x96-Strip-Wells-(Sandwich)

5415