ELISA/assay

Human Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) ELISA Kit

MBS756402

48-Strip-Wells

470 USD

ELISA Kit

Human Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP) ELISA Kit

Putative ATP-dependent Clp protease proteolytic subunit, mitochondrial (CLPP)

putative ATP-dependent Clp protease proteolytic subunit, mitochondrial; putative ATP-dependent Clp protease proteolytic subunit, mitochondrial; caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; caseinolytic mitochondrial matrix peptidase proteolytic subunit

clpp; fj37a05; wu:fj37a05; zgc:112333; si:dkey-204f11.65

Human

This assay has high sensitivity and excellent specificity for detection of CLPP. No significant cross-reactivity or interference between CLPP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CLPP and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This CLPP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CLPP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: CLPP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CLPP antibody and an CLPP-HRP conjugate. The assay sample and buffer are incubated together with CLPP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CLPP concentration since CLPP from samples and CLPP-HRP conjugate compete for the anti-CLPP antibody binding site. Since the number of sites is limited, as more sites are occupied by CLPP from the sample, fewer sites are left to bind CLPP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CLPP concentration in each sample is interpolated from this standard curve.

66472380

NP_001018520.1

NM_001020684.1

48-Strip-Wells

470 USD

96-Strip-Wells

675