ELISA/assay

Human NF-kappa-B inhibitor delta (NFKBID) ELISA Kit

MBS756385

48-Strip-Wells

470 USD

ELISA Kit

Human NF-kappa-B inhibitor delta (NFKBID) ELISA Kit

NF-kappa-B inhibitor delta (NFKBID)

NF-kappa-B inhibitor delta; NF-kappa-B inhibitor delta; NF-kappa-B inhibitor delta; ikB-delta; ikappaBdelta; I-kappa-B-delta; T-cell activation NFKB-like protein; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, delta; I-kappa-B-delta; IkB-delta; IkappaBdelta; IkappaBNS; T-cell activation NFKB-like protein; TA-NFKBH

NFKBID

NFKBID; NFKBID; TA-NFKBH; IkappaBNS; IKBNS; IkB-delta; IkappaBdelta

IKBD_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of N1-N12. No significant cross-reactivity or interference between N1-N12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between N1-N12 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.

Intended Uses: This N1-N12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human N1-N12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: N1-N12 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-N1-N12 antibody and an N1-N12-HRP conjugate. The assay sample and buffer are incubated together with N1-N12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the N1-N12 concentration since N1-N12 from samples and N1-N12-HRP conjugate compete for the anti-N1-N12 antibody binding site. Since the number of sites is limited, as more sites are occupied by N1-N12 from the sample, fewer sites are left to bind N1-N12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The N1-N12 concentration in each sample is interpolated from this standard curve.

41281709

NP_640332.1

NM_139239.1

15,921 Da

NFKBID: May regulate the expression of IL-2, IL-6, and other cytokines through regulation on NF-kappa-B activity. Functions in the regulation of inflammatory responses. May also regulate TCR- induced negative selection of thymocytes. Belongs to the NF-kappa-B inhibitor family. 3 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 19q13.12. Cellular Component: nucleus. Molecular Function: NF-kappaB binding. Biological Process: inhibition of NF-kappaB transcription factor; negative regulation of T cell differentiation in the thymus; negative regulation of I-kappaB kinase/NF-kappaB cascade; inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675