ELISA/assay

Human Neogenin ELISA Kit

MBS756374

48-Strip-Wells

470 USD

ELISA Kit

Human Neogenin ELISA Kit

Neogenin

neogenin; Neogenin; neogenin; neogenin homolog 1; immunoglobulin superfamily DCC subclass member 2; neogenin 1; Immunoglobulin superfamily DCC subclass member 2

NEO

NEO1; NEO1; NGN; IGDCC2; NTN1R2; IGDCC2; NGN

NEO1_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This NAADP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NAADP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: NAADP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NAADP antibody and an NAADP-HRP conjugate. The assay sample and buffer are incubated together with NAADP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NAADP concentration since NAADP from samples and NAADP-HRP conjugate compete for the anti-NAADP antibody binding site. Since the number of sites is limited, as more sites are occupied by NAADP from the sample, fewer sites are left to bind NAADP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAADP concentration in each sample is interpolated from this standard curve.

2078518

AAC51287.1

158,827 Da

Axon Guidance Pathway (105688); CDO In Myogenesis Pathway (160998); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Developmental Biology Pathway (477129); Myogenesis Pathway (160997); Netrin-1 Signaling Pathway (160999)

This gene encodes a cell surface protein that is a member of the immunoglobulin superfamily. The encoded protein consists of four N-terminal immunoglobulin-like domains, six fibronectin type III domains, a transmembrane domain and a C-terminal internal domain that shares homology with the tumor suppressor candidate gene DCC. This protein may be involved in cell growth and differentiation and in cell-cell adhesion. Defects in this gene are associated with cell proliferation in certain cancers. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]

NEO1: May be involved as a regulatory protein in the transition of undifferentiated proliferating cells to their differentiated state. May also function as a cell adhesion molecule in a broad spectrum of embryonic and adult tissues. Belongs to the immunoglobulin superfamily. DCC family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Receptor, misc. Chromosomal Location of Human Ortholog: 15q22.3-q23. Cellular Component: integral to plasma membrane; plasma membrane. Molecular Function: cadherin binding; receptor activity. Biological Process: axon guidance; iron ion homeostasis; muscle cell differentiation; regulation of transcription, DNA-dependent; positive regulation of muscle cell differentiation; cell adhesion; myoblast fusion

48-Strip-Wells

470 USD

96-Strip-Wells

675