ELISA/assay

Mouse Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

MBS756370

48-Strip-Wells

470 USD

ELISA Kit

Mouse Secreted Frizzled Related Protein 1 (SFRP1) ELISA Kit

Secreted Frizzled Related Protein 1 (SFRP1)

secreted frizzled-related protein 1; Secreted frizzled-related protein 1; secreted frizzled-related protein 1; secreted frizzled-related sequence protein 1; secreted frizzled-related protein 1

SFRP1

Sfrp1; Sfrp1; sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik; sFRP-1

SFRP1_MOUSE

Mouse

This assay has high sensitivity and excellent specificity for detection of SFRP-1. No significant cross-reactivity or interference between SFRP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SFRP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogena. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This SFRP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SFRP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay SFRP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SFRP-1 antibody and an SFRP-1-HRP conjugate. The assay sample and buffer are incubated together with SFRP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SFRP-1 concentration since SFRP-1 from samples and SFRP-1-HRP conjugate compete for the anti-SFRP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SFRP-1 from the sample, fewer sites are left to bind SFRP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SFRP-1 concentration in each sample is interpolated from this standard curve.

65301472

NP_038862.2

NM_013834.3

Q8C4U3

35,413 Da

Wnt Signaling Pathway NetPath (198351); Wnt Signaling Pathway (83258); Wnt Signaling Pathway (471)

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; plasma membrane; extracellular region; intracellular; cytosol. Molecular Function: heparin binding; Wnt-protein binding; identical protein binding; protein binding; frizzled binding; cysteine-type endopeptidase activity; drug binding. Biological Process: neural tube development; negative regulation of Wnt receptor signaling pathway; somatic stem cell maintenance; positive regulation of apoptosis; multicellular organismal development; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; proteolysis; response to organic cyclic substance; negative regulation of insulin secretion; negative regulation of BMP signaling pathway; anterior/posterior pattern formation; negative regulation of ossification; positive regulation of smoothened signaling pathway; negative regulation of osteoblast differentiation; female gonad development; response to drug; negative regulation of peptidyl-tyrosine phosphorylation; somitogenesis; digestive tract morphogenesis; positive regulation of cell growth; negative regulation of fibroblast proliferation; negative regulation of bone remodeling; positive regulation of fat cell differentiation; negative regulation of osteoblast proliferation; neural crest cell fate commitment; hemopoietic progenitor cell differentiation; negative regulation of transcription, DNA-dependent; regulation of ossification; actin cytoskeleton organization and biogenesis; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of osteoclast differentiation; dorsal/ventral axis specification; negative regulation of cell proliferation; positive regulation of cell proliferation; hemopoiesis; cell differentiation; negative regulation of cell migration; positive regulation of Wnt receptor signaling pathway; negative regulation of epithelial cell proliferation; negative regulation of B cell differentiation; negative regulation of JNK activity; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; male gonad development; development of primary male sexual characteristics; neural tube closure; negative regulation of cell growth

48-Strip-Wells

470 USD

96-Strip-Wells

675