ELISA/assay

Human Olfactory receptor 10A2 (OR10A2) ELISA Kit

MBS756369

96 Strip Wells

675 USD

ELISA Kit

Human Olfactory receptor 10A2 (OR10A2) ELISA Kit

Olfactory receptor 10A2 (OR10A2)

olfactory receptor 10A2; Olfactory receptor 10A2; olfactory receptor 10A2; hP4 olfactory receptor; olfactory receptor OR11-86; olfactory receptor OR11-82 pseudogene; olfactory receptor, family 10, subfamily A, member 2 pseudogene; olfactory receptor, family 10, subfamily A, member 2; HP4; Olfactory receptor OR11-86

OR10A2

OR10A2; OR10A2; OST363; OR10A2P; OR11-82; OR11-86; OR10A2P

O10A2_HUMAN

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This 2, 5-OAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 2, 5-OAS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This 2, 5-OAS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human 2, 5-OAS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Neurobiology

Principle of the Assay: 2, 5-OAS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-2, 5-OAS antibody and an 2, 5-OAS-HRP conjugate. The assay sample and buffer are incubated together with 2, 5-OAS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the 2, 5-OAS concentration since 2, 5-OAS from samples and 2, 5-OAS-HRP conjugate compete for the anti-2, 5-OAS antibody binding site. Since the number of sites is limited, as more sites are occupied by 2, 5-OAS from the sample, fewer sites are left to bind 2, 5-OAS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 2, 5-OAS concentration in each sample is interpolated from this standard curve.

52317178

NP_001004460.1

NM_001004460.1

33,817 Da

GPCR Downstream Signaling Pathway 119548!!GPCRs, Other Pathway 198765!!Olfactory Signaling Pathway 106383!!Olfactory Transduction Pathway 83087!!Olfactory Transduction Pathway 498!!Signal Transduction Pathway 477114!!Signaling By GPCR Pathway 106356

Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

96 Strip Wells

675 USD