Canine Glycophorin A ELISA Kit | MyBioSource MBS756356 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Glycophorin A ELISA Kit

catalog :

MBS756356

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS756356

products type :

ELISA Kit

products full name :

Canine Glycophorin A ELISA Kit

products short name :

Glycophorin A

other names :

glycophorin-A; Glycophorin-A; glycophorin-A; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Erik; glycophorin A, GPA; MN sialoglycoprotein; glycophorin Sta type C; sialoglycoprotein alpha; Mi.V glycoprotein (24 AA); glycophorin A (MN blood group); recombinant glycophorin A-B Miltenberger-DR; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MNS blood group); MN sialoglycoprotein; PAS-2; Sialoglycoprotein alpha; CD_antigen: CD235a

products gene name :

GYPA

other gene names :

GYPA; GYPA; MN; GPA; MNS; GPSAT; PAS-2; CD235a; GPErik; HGpMiV; HGpMiXI; HGpSta(C); GPA

uniprot entry name :

GLPA_HUMAN

reactivity :

Canine

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This GPA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine GPA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the Assay: GPA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GPA antibody and an GPA-HRP conjugate. The assay sample and buffer are incubated together with GPA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GPA concentration since GPA from samples and GPA-HRP conjugate compete for the anti-GPA antibody binding site. Since the number of sites is limited, as more sites are occupied by GPA from the sample, fewer sites are left to bind GPA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GPA concentration in each sample is interpolated from this standard curve.

ncbi gi num :

298286506

ncbi acc num :

NP_002090.4

ncbi gb acc num :

NM_002099.6

ncbi mol weight :

13,000 Da

ncbi pathways :

Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]

uniprot summary :

GYPA: Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV). Belongs to the glycophorin A family. Protein type: Adaptor/scaffold; Membrane protein, integral. Chromosomal Location of Human Ortholog: 4q31.21. Cellular Component: membrane; integral to plasma membrane; plasma membrane; external side of plasma membrane. Molecular Function: viral receptor activity; identical protein binding; protein homodimerization activity. Biological Process: entry of virus into host cell; cytoskeletal anchoring; regulation of response to osmotic stress. Disease: Malaria, Susceptibility To

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage