ELISA/assay

Monkey CD40 ELISA Kit

MBS756327

48-Strip-Wells

470 USD

ELISA Kit

Monkey CD40 ELISA Kit

CD40

CD40, partial; CD40; CD40

CD40

Q09LL4_HUMAN

Monkey

This assay has high sensitivity and excellent specificity for detection of CD40. No significant cross-reactivity or interference between CD40 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CD40 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Immunology

Intended Uses: This CD40 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey CD40. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay CD40 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CD40 antibody and an CD40-HRP conjugate. The assay sample and buffer are incubated together with CD40-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD40 concentration since CD40 from samples and CD40-HRP conjugate compete for the anti-CD40 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD40 from the sample, fewer sites are left to bind CD40-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD40 concentration in each sample is interpolated from this standard curve.

114053977

ABI49511.1

13,158 Da

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

48-Strip-Wells

470 USD

96-Strip-Wells

675