ELISA/assay

Mouse Epididymis Protein 4 ELISA Kit

MBS756249

48-Strip-Wells

470 USD

ELISA Kit

Mouse Epididymis Protein 4 ELISA Kit

Epididymis Protein 4

WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; epididymal protein 4; epididymal secretory protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; major epididymis-specific protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; WAP four-disulfide core domain 2; Epididymal secretory protein E4; Major epididymis-specific protein E4; Putative protease inhibitor WAP5

HE-4

WFDC2; WFDC2; HE4; WAP5; EDDM4; dJ461P17.6; HE4; WAP5

WFDC2_HUMAN

Mouse

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This HE4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HE4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Intended Uses: This HE-4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HE-4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: HE-4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HE-4 antibody and an HE-4-HRP conjugate. The assay sample and buffer are incubated together with HE-4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HE-4 concentration since HE-4 from samples and HE-4-HRP conjugate compete for the anti-HE-4 antibody binding site. Since the number of sites is limited, as more sites are occupied by HE-4 from the sample, fewer sites are left to bind HE-4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HE-4 concentration in each sample is interpolated from this standard curve.

56699495

NP_006094.3

NM_006103.3

11,043 Da

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space. Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity. Biological Process: spermatogenesis; proteolysis

48-Strip-Wells

470 USD

96-Strip-Wells

675