catalog number :
MBS756236
products type :
ELISA Kit
products full name :
Human Malonyl CoA ELISA Kit
products short name :
Malonyl CoA
other names :
malonyl-CoA; Malonyl CoA-acyl carrier protein transacylase
products gene name :
Malonyl CoA
other gene names :
fabD; SENTW_2059
uniprot entry name :
E9A321_SALET
specificity :
This assay has high sensitivity and excellent specificity for detection of M-COA. No significant cross-reactivity or interference between M-COA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between M-COA and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.
products description :
Intended Uses: This M-COA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human M-COA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: M-COA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-M-COA antibody and an M-COA-HRP conjugate. The assay sample and buffer are incubated together with M-COA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the M-COA concentration since M-COA from samples and M-COA-HRP conjugate compete for the anti-M-COA antibody binding site. Since the number of sites is limited, as more sites are occupied by M-COA from the sample, fewer sites are left to bind M-COA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The M-COA concentration in each sample is interpolated from this standard curve.
ncbi acc num :
YP_006886348.1
ncbi gb acc num :
NT_187103.1
ncbi mol weight :
32,405 Da