catalog number :
MBS756229
products type :
ELISA Kit
products full name :
Monkey Tetranectin (CLEC3B) ELISA Kit
products short name :
Tetranectin (CLEC3B)
other names :
tetranectin; Tetranectin; tetranectin; plasminogen kringle 4-binding protein; tetranectin (plasminogen binding protein); tetranectin (plasminogen-binding protein); C-type lectin domain family 3, member B; C-type lectin domain family 3 member B; Plasminogen kringle 4-binding protein
products gene name :
TN (CLEC3B)
other gene names :
CLEC3B; CLEC3B; TN; TNA; TNA; TN
uniprot entry name :
TETN_HUMAN
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
products description :
Intended Uses: This TP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey TP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: TP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TP antibody and an TP-HRP conjugate. The assay sample and buffer are incubated together with TP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TP concentration since TP from samples and TP-HRP conjugate compete for the anti-TP antibody binding site. Since the number of sites is limited, as more sites are occupied by TP from the sample, fewer sites are left to bind TP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TP concentration in each sample is interpolated from this standard curve.
ncbi acc num :
NP_003269.2
ncbi gb acc num :
NM_003278.2
ncbi mol weight :
22,537 Da
uniprot summary :
CLEC3B: Tetranectin binds to plasminogen and to isolated kringle 4. May be involved in the packaging of molecules destined for exocytosis. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3p22-p21.3. Cellular Component: extracellular matrix; extracellular space; granular component; cytoplasm; extracellular region. Molecular Function: heparin binding; calcium ion binding; carbohydrate binding. Biological Process: ossification; skeletal development; bone mineralization