ELISA/assay

Sheep Anti-Endothelin 1 ELISA Kit

MBS756221

48-Strip-Wells

470 USD

ELISA Kit

Sheep Anti-Endothelin 1 ELISA Kit

Anti-Endothelin 1

endothelin-1; Endothelin-1; endothelin-1; ET-1; preproendothelin-1; gene for endothelin; Preproendothelin-1; PPET1

ET-1

EDN1; EDN1; PPET1; ET-1; PPET1

EDN1_CANFA

Sheep

This assay has high sensitivity and excellent specificity for detection of ET1-Ab. No significant cross-reactivity or interference between ET1-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ET1-Ab and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This ET1-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep ET1-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Epigenetics and Nuclear Signaling

Principle of the assay: ET1-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Endothelin 1 antigen and an ET1-Ab-HRP conjugate. The assay sample and buffer are incubated together with ET1-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ET1-Ab concentration since ET1-Ab from samples and ET1-Ab-HRP conjugate compete for the Endothelin 1 antigen binding site. Since the number of sites is limited, as more sites are occupied by ET1-Ab from the sample, fewer sites are left to bind ET1-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ET1-Ab concentration in each sample is interpolated from this standard curve.

50950175

NP_001002956.1

NM_001002956.1

22,977 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1017189); G Alpha (q) Signalling Events Pathway (1017222); GPCR Downstream Signaling Pathway (1017218); GPCR Ligand Binding Pathway (1017188); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1017237); HIF-1 Signaling Pathway (695209); Melanogenesis Pathway (84142); Melanogenesis Pathway (504); Peptide Ligand-binding Receptors Pathway (1017190); Signal Transduction Pathway (1017095)

48-Strip-Wells

470 USD

96-Strip-Wells

675