catalog number :
MBS756213
products type :
ELISA Kit
products full name :
Mouse Heparin-Platelet Factor 4 antibody ELISA Kit
products short name :
Heparin-Platelet Factor 4 antibody
products gene name :
HPF4Ab
specificity :
This assay has high sensitivity and excellent specificity for detection of HPF4Ab. No significant cross-reactivity or interference between HPF4Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete thecross-reactivity detection between HPF4Ab and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This HPF4Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse HPF4Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the assay: HPF4Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Heparin Platelet Factor 4 antigen and an HPF4Ab-HRP conjugate. The assay sample and buffer are incubated together with HPF4Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HPF4Ab concentration since HPF4Ab from samples and HPF4Ab-HRP conjugate compete for the Heparin Platelet Factor 4 antigen binding site. Since the number of sites is limited, as more sites are occupied by HPF4Ab from the sample, fewer sites are left to bind HPF4Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HPF4Ab concentration in each sample is interpolated from this standard curve.