Human Dentin Matrix Protein 1 ELISA Kit | MyBioSource MBS756165 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Dentin Matrix Protein 1 ELISA Kit

catalog :

MBS756165

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS756165

products type :

ELISA Kit

products full name :

Human Dentin Matrix Protein 1 ELISA Kit

products short name :

Dentin Matrix Protein 1

other names :

dentin matrix acidic phosphoprotein 1 isoform 1; Dentin matrix acidic phosphoprotein 1; dentin matrix acidic phosphoprotein 1; dentin matrix protein 1; dentin matrix acidic phosphoprotein 1

products gene name :

DMP1

other gene names :

DMP1; DMP1; ARHP; ARHR; DMP-1; DMP-1; Dentin matrix protein 1

uniprot entry name :

DMP1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of DMP-1. No significant cross-reactivity or interference between DMP-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DMP-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This DMP-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DMP-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: DMP-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DMP-1 antibody and an DMP-1-HRP conjugate. The assay sample and buffer are incubated together with DMP-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DMP-1 concentration since DMP-1 from samples and DMP-1-HRP conjugate compete for the anti-DMP-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by DMP-1 from the sample, fewer sites are left to bind DMP-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DMP-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4758172

ncbi acc num :

NP_004398.1

ncbi gb acc num :

NM_004407.3

ncbi mol weight :

54,115 Da

ncbi pathways :

ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

DMP1: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite. Defects in DMP1 are the cause of rickets hypophosphatemic autosomal recessive type 1 (ARHR1). A hereditary form of hypophosphatemic rickets, a disorder of proximal renal tubule function that causes phosphate loss, hypophosphatemia and skeletal deformities, including rickets and osteomalacia unresponsive to vitamin D. Symptoms are bone pain, fractures and growth abnormalities. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q21. Cellular Component: nucleoplasm; proteinaceous extracellular matrix; intracellular membrane-bound organelle; cytoplasm; nucleolus; extracellular region; nucleus. Molecular Function: integrin binding; extracellular matrix binding; calcium ion binding. Biological Process: extracellular matrix organization and biogenesis; ossification; biomineral formation. Disease: Hypophosphatemic Rickets, Autosomal Recessive, 1

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage