catalog number :
MBS755994
products type :
ELISA Kit
products full name :
Mouse Acetylcholine Receptor ELISA Kit
products short name :
Acetylcholine Receptor
other names :
acetylcholine receptor, partial; Acetylcholine receptor; Acetylcholine receptor
products gene name :
AChR
uniprot entry name :
Q13695_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of AchR. No significant cross-reactivity or interference between AchR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AchR and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This AchR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse AchR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: AchR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AchR antibody and an AchR-HRP conjugate. The assay sample and buffer are incubated together with AchR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AchR concentration since AchR from samples and AchR-HRP conjugate compete for the anti-AchR antibody binding site. Since the number of sites is limited, as more sites are occupied by AchR from the sample, fewer sites are left to bind AchR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AchR concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAA86792.1
ncbi mol weight :
1,282 Da