product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Bovine Obestatin ELISA Kit
catalog :
MBS755984
quantity :
48-Strip-Wells
price :
440 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS755984
products type :
ELISA Kit
products full name :
Bovine Obestatin ELISA Kit
products short name :
[Obestatin]
products gene name :
[Obestatin]
reactivity :
Bovine
specificity :
The microplates are coated with highly purified native human nucleosomes. No crossreactivities to other autoantigens have been found. The AESKULISA Nucleo-h Test shows a specificity of 90%. The sensitivity of nucleosome specific antibodies is 84-88% in SLE.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Human Serum. Sensitivity: 1.0 U/ml.
products description :
Intended Uses: This Nucleo-h IgG ELISA is a solid phase enzyme immunoassay with human native nucleosomes isolated from the eukaryotic cell line HeLa for the quantitative and qualitative detection of antibodies against nucleosomes and their components dsDNA and histones in human serum. For research use only, not for use in diagnostic procedures. Principle of the Assay: Serum samples diluted 1:101 are incubated in the microplates coated with the specific antigen. Antibodies, if present in the specimen, bind to the antigen. The unbound fraction is washed off in the following step. Afterwards anti-human immunoglobulins conjugated to horseradish peroxidase (conjugate) are incubated and react with the antigen-antibody complex of the samples in the microplates. Unbound conjugate is washed off in the following step. Addition of TMB-substrate generates an enzymatic colorimetric (blue) reaction, which is stopped by diluted acid (color changes to yellow). The intensity of color formation from the chromogen is a function of the amount of conjugate bound to the antigen-antibody complex and this is proportional to the initial concentration of the respective antibodies in the sample!!Background/Introduction: Nucleosomes are the basic structure of chromatin important for the compaction of DNA in the nucleus. A nucleosome comprises of four histones H2A, H2B, H3 and H4 forming an octamer and 146 bp of dsDNA wrapped 1 ¾ turns around the core (H2A, H2B, H3, H4)2 octamer. Histone H1 interacts with the nucleosome and together with linked-DNA connects neighboring nucleosomes.
size1 :
48-Strip-Wells
price1 :
440 USD
size2 :
96-Strip-Wells
price2 :
640
size3 :
5x96-Strip-Wells
price3 :
2895
size4 :
10x96-Strip-Wells
price4 :
5415
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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