ELISA/assay

Bovine Regenerating islet-derived protein 3-gamma ELISA Kit

MBS755934

48-Strip-Wells

470 USD

ELISA Kit

Bovine Regenerating islet-derived protein 3-gamma ELISA Kit

Regenerating islet-derived protein 3-gamma

regenerating islet-derived protein 3-gamma isoform 1; Regenerating islet-derived protein 3-gamma; regenerating islet-derived protein 3-gamma; REG-3-gamma; reg III-gamma; regenerating gene III; pancreatitis-associated protein 1B; pancreatitis-associated protein IB; regenerating islet-derived protein III-gamma; regenerating islet-derived 3 gamma; Pancreatitis-associated protein 1B; PAP-1B; Pancreatitis-associated protein IB; PAP IB; Regenerating islet-derived protein III-gamma; REG III; Reg III-gamma

REG3G

REG3G; REG3G; PAP1B; PAPIB; PAP IB; PAP-1B; UNQ429; LPPM429; REG III; REG-III; PAP1B; UNQ429/PRO162; REG-3-gamma; PAP-1B; PAP IB; REG III; Reg III-gamma

REG3G_HUMAN

Bovine

This assay has high sensitivity and excellent specificity for detection of REG-3?. No significant cross-reactivity or interference between REG-3? and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between REG-3? and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This REG-3? ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine REG-3?. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cell Biology

Principle of the assay: REG-3? ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-REG-3? antibody and an REG-3?-HRP conjugate. The assay sample and buffer are incubated together with REG-3?-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the REG-3? concentration since REG-3? from samples and REG-3?-HRP conjugate compete for the anti-REG-3? antibody binding site. Since the number of sites is limited, as more sites are occupied by REG-3? from the sample, fewer sites are left to bind REG-3?-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The REG-3? concentration in each sample is interpolated from this standard curve.

56699413

NP_001008388.1

NM_001008387.2

14,409 Da

This gene encodes a member of the regenerating islet-derived genes (REG)3 protein family. These proteins are secreted, C-type lectins with a carbohydrate recognition domain and N-terminal signal peptide. The protein encoded by this gene is an antibacterial lectin with activity against Gram-positive bacteria. Alternative splicing results in multiple transcript variants encoding multiple isoforms. [provided by RefSeq, Jun 2012]

REG3G: Might be a stress protein involved in the control of bacterial proliferation. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p12. Cellular Component: cytoplasm; extracellular region. Molecular Function: carbohydrate binding. Biological Process: MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of keratinocyte differentiation; acute-phase response

48-Strip-Wells

470 USD

96-Strip-Wells

675