Rat Epididymis Protein 4 ELISA Kit | MyBioSource MBS755820 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Epididymis Protein 4 ELISA Kit

catalog :

MBS755820

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS755820

products type :

ELISA Kit

products full name :

Rat Epididymis Protein 4 ELISA Kit

products short name :

Epididymis Protein 4

other names :

WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; epididymal protein 4; epididymal secretory protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; major epididymis-specific protein E4; epididymis-specific, whey-acidic protein type, four-disulfide core; WAP four-disulfide core domain 2; Epididymal secretory protein E4; Major epididymis-specific protein E4; Putative protease inhibitor WAP5

products gene name :

HE-4

other gene names :

WFDC2; WFDC2; HE4; WAP5; EDDM4; dJ461P17.6; HE4; WAP5

uniprot entry name :

WFDC2_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of RE4. No significant cross-reactivity or interference between RE4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RE4 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pmol/ml.

other info2 :

Intended Uses: This RE4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat RE4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: RE4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RE4 antibody and an RE4-HRP conjugate. The assay sample and buffer are incubated together with RE4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RE4 concentration since RE4 from samples and RE4-HRP conjugate compete for the anti-RE4 antibody binding site. Since the number of sites is limited, as more sites are occupied by RE4 from the sample, fewer sites are left to bind RE4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RE4 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

56699495

ncbi acc num :

NP_006094.3

ncbi gb acc num :

NM_006103.3

ncbi mol weight :

11,043 Da

ncbi summary :

This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]

uniprot summary :

WFDC2: a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. [provided by RefSeq, Jul 2008]. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q13.12. Cellular Component: extracellular space. Molecular Function: serine-type endopeptidase inhibitor activity; aspartic-type endopeptidase inhibitor activity; endopeptidase inhibitor activity; cysteine protease inhibitor activity. Biological Process: spermatogenesis; proteolysis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage