Human 1, 5 anhydro D fructose reductase (AKR1CL2) ELISA Kit | MyBioSource MBS755705 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 1, 5 anhydro D fructose reductase (AKR1CL2) ELISA Kit

catalog :

MBS755705

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS755705

products type :

ELISA Kit

products full name :

Human 1, 5 anhydro D fructose reductase (AKR1CL2) ELISA Kit

products short name :

1, 5 anhydro D fructose reductase (AKR1CL2)

other names :

glutamate decarboxylase 2; Glutamate decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform; glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform

products gene name :

AKR1CL2

other gene names :

GAD2; GAD2; GAD65; GAD65; GAD-65

uniprot entry name :

DCE2_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of GAD65-Ab. No significant cross-reactivity or interference between GAD65-Ab and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GAD65-Ab and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL

other info2 :

Intended Uses: This GAD65-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human GAD65-Ab. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: GAD65-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Glutamic Acid Decarboxylase antigen and an GAD65-Ab-HRP conjugate. The assay sample and buffer are incubated together with GAD65-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GAD65-Ab concentration since GAD65-Ab from samples and GAD65-Ab-HRP conjugate compete for the Glutamic Acid Decarboxylase antigen binding site. Since the number of sites is limited, as more sites are occupied by GAD65-Ab from the sample, fewer sites are left to bind GAD65-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GAD65-Ab concentration in each sample is interpolated from this standard curve.

ncbi gi num :

197276620

ncbi acc num :

NP_001127838.1

ncbi gb acc num :

NM_001134366.1

ncbi mol weight :

65,411 Da

ncbi pathways :

Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Biogenic Amine Synthesis Pathway (198793); Butanoate Metabolism Pathway (83007); Butanoate Metabolism Pathway (391); GABA (gamma-Aminobutyrate) Shunt Pathway (413433); GABA (gamma-Aminobutyrate) Shunt Pathway (468220); GABA Synthesis Pathway (187175); GABA Synthesis, Release, Reuptake And Degradation Pathway (187174)

ncbi summary :

This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]

uniprot summary :

GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family. Protein type: EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - butanoate; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - taurine and hypotaurine; Lyase. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; perinuclear region of cytoplasm; axon; plasma membrane; cytosol; cell junction. Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding. Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage