Rat Lipoxin A4 ELISA Kit | MyBioSource MBS755644 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Lipoxin A4 ELISA Kit

catalog :

MBS755644

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS755644

products type :

ELISA Kit

products full name :

Rat Lipoxin A4 ELISA Kit

products short name :

Lipoxin A4

other names :

lipoxin A4 receptor; N-formyl peptide receptor 2; N-formyl peptide receptor 2; RFP; FMLP-R-I; LXA4 receptor; FMLP-related receptor I; formyl peptide receptor-like 1; lipoxin A4 receptor (formyl peptide receptor related); formyl peptide receptor 2; FMLP-related receptor I; FMLP-R-I; Formyl peptide receptor-like 1; HM63; Lipoxin A4 receptor; LXA4 receptor; RFP

products gene name :

LXA4

other gene names :

FPR2; FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II; FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor

uniprot entry name :

FPR2_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of LXA4. No significant cross-reactivity or interference between LXA4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between LXA4 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This LXA4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat LXA4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: LXA4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LXA4 antibody and an LXA4-HRP conjugate. The assay sample and buffer are incubated together with LXA4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LXA4 concentration since LXA4 from samples and LXA4-HRP conjugate compete for the anti-LXA4 antibody binding site. Since the number of sites is limited, as more sites are occupied by LXA4 from the sample, fewer sites are left to bind LXA4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LXA4 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

1916076

ncbi acc num :

AAB51133.1

ncbi mol weight :

38,964 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (161021); G Alpha (i) Signalling Events Pathway (119550); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); GPCR Ligand Binding Pathway (161020); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (645295); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

uniprot summary :

FPR2: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; N-formyl peptide receptor activity. Biological Process: G-protein coupled receptor protein signaling pathway; inflammatory response; cell adhesion; chemotaxis; cell motility

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage