ELISA/assay

Sheep Retinoic Acid Early Transcript 1L ELISA Kit

MBS755206

48-Strip-Wells

470 USD

ELISA Kit

Sheep Retinoic Acid Early Transcript 1L ELISA Kit

Retinoic Acid Early Transcript 1L

retinoic acid early transcript 1L protein; Retinoic acid early transcript 1L protein; retinoic acid early transcript 1L protein; UL16 binding protein 6; retinoic acid early transcript 1L

RAET1L

RAET1L; RAET1L; ULBP6

RET1L_HUMAN

Sheep

This assay has high sensitivity and excellent specificity for detection of RAET1L. No significant cross-reactivity or interference between RAET1L and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RAET1L and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This RAET1L ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep RAET1L. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: RAET1L ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RAET1L antibody and an RAET1L-HRP conjugate. The assay sample and buffer are incubated together with RAET1L-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RAET1L concentration since RAET1L from samples and RAET1L-HRP conjugate compete for the anti-RAET1L antibody binding site. Since the number of sites is limited, as more sites are occupied by RAET1L from the sample, fewer sites are left to bind RAET1L-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RAET1L concentration in each sample is interpolated from this standard curve.

126723295

NP_570970.2

NM_130900.2

27,509 Da

Natural Killer Cell Mediated Cytotoxicity Pathway (83079); Natural Killer Cell Mediated Cytotoxicity Pathway (490)

RAET1L belongs to the RAET1 family of major histocompatibility complex (MHC) class I-related genes, which are located within a 180-kb cluster on chromosome 6q24.2-q25.3. The REAT1 genes encode glycoproteins that contain extracellular alpha-1 and alpha-2 domains, but they lack the membrane proximal Ig-like alpha-3 domain. Most RAET1 glycoproteins are anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage (Radosavljevic et al., 2002 [PubMed 11827464]).[supplied by OMIM, Mar 2008]

RAET1L: Belongs to the MHC class I family. Protein type: Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 6q25.1. Cellular Component: plasma membrane. Molecular Function: peptide antigen binding; natural killer cell lectin-like receptor binding. Biological Process: antigen processing and presentation of peptide antigen via MHC class I; natural killer cell mediated cytotoxicity

48-Strip-Wells

470 USD

96-Strip-Wells

675