Monkey Interleukin 29 ELISA Kit | MyBioSource MBS755121 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Interleukin 29 ELISA Kit

catalog :

MBS755121

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS755121

products type :

ELISA Kit

products full name :

Monkey Interleukin 29 ELISA Kit

products short name :

Interleukin 29

other names :

interleukin 29; Interferon lambda-1; interferon lambda-1; IFN-lambda-1; interleukin-29; cytokine Zcyto21; interferon-lambda-1; interleukin 29 (interferon, lambda 1); interferon, lambda 1; Cytokine Zcyto21; Interleukin-29; IL-29

products gene name :

IL-29

other gene names :

IFNL1; IFNL1; IL29; IL-29; IL29; ZCYTO21; IFN-lambda-1; IL-29

uniprot entry name :

IFNL1_HUMAN

reactivity :

Monkey

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This IL-29 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IL-29. This ELISA kit for research use only, not for therapeutic or diagnostic applications

products categories :

Immunology

products description :

Principle of the Assay: IL-29 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-29 antibody and an IL-29-HRP conjugate. The assay sample and buffer are incubated together with IL-29-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-29 concentration since IL-29 from samples and IL-29-HRP conjugate compete for the anti-IL-29 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-29 from the sample, fewer sites are left to bind IL-29-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-29 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

38569954

ncbi acc num :

AAR24511.1

ncbi mol weight :

21,898 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Type III Interferon Signaling Pathway (755437)

ncbi summary :

This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28A (IL28A), and interleukin 28B (IL28B) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). [provided by RefSeq, Jul 2008]

uniprot summary :

IL29: Cytokine with immunomodulatory activity. May play a role in antiviral immunity. Up-regulates MHC class I antigen expression. Ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IL28RA. The ligand/receptor complex seems to signal through the Jak-STAT pathway. Belongs to the IL-28/IL-29 family. Protein type: Secreted; Cytokine; Cell cycle regulation; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19q13.13. Cellular Component: extracellular space; extracellular region; interleukin-28 receptor complex. Molecular Function: cytokine activity; interleukin-28 receptor binding; receptor binding. Biological Process: positive regulation of immune response; positive regulation of transcription, DNA-dependent; positive regulation of JAK-STAT cascade; JAK-STAT cascade; negative regulation of T cell differentiation; negative regulation of cell proliferation; negative regulation of interleukin-13 production; positive regulation of interferon-gamma production; negative regulation of T-helper 2 type immune response; positive regulation of tyrosine phosphorylation of STAT protein; negative regulation of interleukin-5 production; positive regulation of MHC class I biosynthetic process; negative regulation of transcription, DNA-dependent; negative regulation of memory T cell differentiation; defense response to virus

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage