ELISA/assay

Mouse Ferroportin ELISA Kit

MBS755115

48-Strip-Wells

470 USD

ELISA Kit

Mouse Ferroportin ELISA Kit

Ferroportin

ferroportin 1 variant I, partial; Solute carrier family 40 member 1; solute carrier family 40 member 1; iron regulated gene 1; putative ferroportin 1 variant IIIA; putative ferroportin 1 variant IIIB; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; solute carrier family 40 (iron-regulated transporter), member 1; Ferroportin-1; Iron-regulated transporter 1

FPN

SLC40A1; SLC40A1; FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3; FPN1; IREG1; SLC11A3; MSTP079

S40A1_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of FPN. No significant cross-reactivity or interference between FPN and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FPN and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL

Intended Uses: This FPN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse FPN. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FPN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FPN antibody and an FPN-HRP conjugate. The assay sample and buffer are incubated together with FPN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FPN concentration since FPN from samples and FPN-HRP conjugate compete for the anti-FPN antibody binding site. Since the number of sites is limited, as more sites are occupied by FPN from the sample, fewer sites are left to bind FPN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FPN concentration in each sample is interpolated from this standard curve.

67633296

AAY78556.1

62,542 Da

Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008]

SLC40A1: May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4). HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation. Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily. Protein type: Transporter, SLC family; Transporter; Membrane protein, multi-pass; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q32. Cellular Component: multivesicular body; synaptic vesicle; integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; integral to membrane; intracellular. Molecular Function: protein binding; iron ion transmembrane transporter activity. Biological Process: anatomical structure morphogenesis; cellular iron ion homeostasis; positive regulation of transcription from RNA polymerase II promoter; lymphocyte homeostasis; transmembrane transport; negative regulation of apoptosis. Disease: Hemochromatosis, Type 4

48-Strip-Wells

470 USD

96-Strip-Wells

675