catalog number :
MBS754865
products type :
ELISA Kit
products full name :
Human S adenosyl L methionine ELISA Kit
products short name :
S adenosyl L methionine
other names :
S-adenosyl-L-methionine; S-adenosyl-L-methionine; S-adenosyl-L-methionine
uniprot entry name :
S7QY86_9MYCO
specificity :
This assay has high sensitivity and excellent specificity for detection of SA-LM. No significant cross-reactivity or interference between SA-LM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SA-LM and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This SA-LM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SA-LM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: SA-LM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SA-LM antibody and an SA-LM-HRP conjugate. The assay sample and buffer are incubated together with SA-LM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SA-LM concentration since SA-LM from samples and SA-LM-HRP conjugate compete for the anti-SA-LM antibody binding site. Since the number of sites is limited, as more sites are occupied by SA-LM from the sample, fewer sites are left to bind SA-LM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SA-LM concentration in each sample is interpolated from this standard curve.
ncbi acc num :
WP_020787021.1
ncbi gb acc num :
WP_020787021.1
ncbi mol weight :
39,097 Da