ELISA/assay

Rat Soluble IL-1 Receptor 4 ELISA Kit

MBS754161

48-Strip-Wells

470 USD

ELISA Kit

Rat Soluble IL-1 Receptor 4 ELISA Kit

Soluble IL-1 Receptor 4

ST2, partial; Interleukin-1 receptor-like 1

IL1RL1; DER4; T1

ILRL1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of sIL1R4/ST2. No significant cross-reactivity or interference between sIL1R4/ST2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between sIL1R4/ST2 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This sIL1R4/ST2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat sIL1R4/ST2. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: sIL1R4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-sIL1R4 antibody and an sIL1R4-HRP conjugate. The assay sample and buffer are incubated together with sIL1R4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the sIL1R4 concentration since sIL1R4 from samples and sIL1R4-HRP conjugate compete for the anti-sIL1R4 antibody binding site. Since the number of sites is limited, as more sites are occupied by sIL1R4 from the sample, fewer sites are left to bind sIL1R4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The sIL1R4 concentration in each sample is interpolated from this standard curve.

2217949

BAA20539.1

23,723 Da

IL1RL1: receptor for IL33. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of ERK1 and/or ERK2, p38-alpha, and JNK1. Apparently promotes the activation and differentiation of T-helper 2 cells. Belongs to the interleukin-1 receptor family. Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Three isoforms of the human protein are produced by alternative splicing. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. Protein type: Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2q12. Cellular Component: extracellular matrix; integral to membrane; extracellular region; external side of plasma membrane. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; receptor signaling protein activity; interleukin-1 receptor activity. Biological Process: negative regulation of T-helper 1 type immune response; negative regulation of interferon-gamma production; immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675