ELISA/assay

Rat Phosphorylated Ca2+/calmodulin-dependent protein kinase ELISA Kit

MBS754113

48-Strip-Wells

470 USD

ELISA Kit

Rat Phosphorylated Ca2+/calmodulin-dependent protein kinase ELISA Kit

Phosphorylated Ca2+/calmodulin-dependent protein kinase

P-CaM kinase

Rat

This assay has high sensitivity and excellent specificity for detection of pCa+CAMK. No significant cross-reactivity or interference between pCa+CAMK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between pCa+CAMK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This pCa+CAMK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat pCa+CAMK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Signal Transduction

Principle of the assay: pCa+CAMK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-pCa+CAMK antibody and an pCa+CAMK-HRP conjugate. The assay sample and buffer are incubated together with pCa+CAMK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pCa+CAMK concentration since pCa+CAMK from samples and pCa+CAMK-HRP conjugate compete for the anti-pCa+CAMK antibody binding site. Since the number of sites is limited, as more sites are occupied by pCa+CAMK from the sample, fewer sites are left to bind pCa+CAMK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pCa+CAMK concentration in each sample is interpolated from this standard curve.

48-Strip-Wells

470 USD

96-Strip-Wells

675