product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Porcine Elastase-1 ELISA Kit
catalog :
MBS753924
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS753924
products type :
ELISA Kit
products full name :
Porcine Elastase-1 ELISA Kit
products short name :
Elastase-1
other names :
elastase 1; Chymotrypsin-like elastase family member 1; Elastase-1
products gene name :
ELA1
other gene names :
CELA1; ELA1
uniprot entry name :
CELA1_PIG
reactivity :
Porcine
specificity :
This assay has high sensitivity and excellent specificity for detection of ELA-1. No significant cross-reactivity or interference between ELA-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ELA-1 and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.
other info2 :
Intended Uses: This ELA-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine ELA-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Cell Biology
products description :
Principle of the assay: ELA-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ELA-1 antibody and an ELA-1-HRP conjugate. The assay sample and buffer are incubated together with ELA-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ELA-1 concentration since ELA-1 from samples and ELA-1-HRP conjugate compete for the anti-ELA-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ELA-1 from the sample, fewer sites are left to bind ELA-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ELA-1 concentration in each sample is interpolated from this standard curve.
ncbi gi num :
1942
ncbi acc num :
CAA27670.1
uniprot acc num :
P00772
ncbi mol weight :
28,821 Da
uniprot summary :
CELA1: Acts upon elastin. Protein type: Secreted, signal peptide; EC 3.4.21.36; Secreted; Protease. Cellular Component: extracellular region. Molecular Function: protein binding; metal ion binding; serine-type endopeptidase activity. Biological Process: Wnt receptor signaling pathway; multicellular organism growth; regulation of cell differentiation; exocrine pancreas development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; proteolysis; inflammatory response; post-embryonic development; regulation of cell proliferation
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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