ELISA/assay

Rat Epidermal fatty acid binding protein 5 ELISA Kit

MBS753790

48-Strip-Wells

470 USD

ELISA Kit

Rat Epidermal fatty acid binding protein 5 ELISA Kit

Epidermal fatty acid binding protein 5

epidermal fatty acid binding protein 5; Fatty acid-binding protein, epidermal; fatty acid-binding protein, epidermal; fatty acid-binding protein 5; cutaneous fatty acid-binding protein; epidermal-type fatty acid-binding protein; fatty acid binding protein 5, epidermal; Cutaneous fatty acid-binding protein; C-FABP; DA11; Epidermal-type fatty acid-binding protein; E-FABP; Fatty acid-binding protein 5

Efabp5

Fabp5; Fabp5; DA11; C-FABP; E-FABP; C-FABP; E-FABP

FABP5_RAT

Rat

This assay has high sensitivity and excellent specificity for detection of FABP5. No significant cross-reactivity or interference between FABP5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FABP5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.

Intended Uses: This FABP5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FABP5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Metabolism

Principle of the assay: FABP5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for FABP5. Standards or samples are then added to the microtiter plate wells and FABP5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of FABP5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for FABP5 are added to each well to "sandwich" the FABP5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain FABP5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP5 concentration in each sample is interpolated from this standard curve.

82395853

ABB72486.1

P55053

15,059 Da

PPAR Signaling Pathway (83434); PPAR Signaling Pathway (450)

binds long chain fatty acids [RGD, Feb 2006]

FABP5: High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. Protein type: Lipid-binding. Cellular Component: cytoplasm. Molecular Function: transporter activity; fatty acid binding. Biological Process: transport; phosphatidylcholine biosynthetic process; response to wounding; glucose metabolic process; glucose transport; lipid metabolic process

48-Strip-Wells

470 USD

96-Strip-Wells

675