ELISA/assay

Rat Bone Morphogenetic Protein Receptor 1B ELISA Kit

MBS753333

48-Strip-Wells

470 USD

ELISA Kit

Rat Bone Morphogenetic Protein Receptor 1B ELISA Kit

Bone Morphogenetic Protein Receptor 1B

bone morphogenetic protein receptor type-1B isoform b; Bone morphogenetic protein receptor type-1B; bone morphogenetic protein receptor type-1B; BMPR-1B; BMP type-1B receptor; activin receptor-like kinase 6; serine/threonine receptor kinase; bone morphogenetic protein receptor, type IB; CD_antigen: CDw293

BMPR1B

BMPR1B; BMPR1B; ALK6; ALK-6; CDw293; BMP type-1B receptor; BMPR-1B

BMR1B_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of BMPR1B. No significant cross-reactivity or interference between BMPR1B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between BMPR1B and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This BMPR1B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat BMPR1B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Cardiovascular

. Principle of the assay: BMPR1B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-BMPR1B antibody and an BMPR1B-HRP conjugate. The assay sample and buffer are incubated together with BMPR1B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BMPR1B concentration since BMPR1B from samples and BMPR1B-HRP conjugate compete for the anti-BMPR1B antibody binding site. Since the number of sites is limited, as more sites are occupied by BMPR1B from the sample, fewer sites are left to bind BMPR1B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BMPR1B concentration in each sample is interpolated from this standard curve.

377823731

NP_001243723.1

NM_001256794.1

60,582 Da

BMP Signalling And Regulation Pathway (198910); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); Ovarian Infertility Genes Pathway (198801); Signal Transduction Pathway (477114); Signaling By BMP Pathway (106336); Signaling Pathways Regulating Pluripotency Of Stem Cells (1026136); Signaling Pathways Regulating Pluripotency Of Stem Cells (1033502)

This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

BMPR1B: a kinase of the STKR family. A type I cell-surface receptor for bone morphogenetic protein. Expressed at ighest level in the prostate. May be upregulated by androgen stimulation in cells which expresses the androgen receptor. Protein type: Kinase, protein; EC 2.7.11.30; Protein kinase, Ser/Thr (receptor); Protein kinase, TKL; Membrane protein, integral; TKL group; STKR family; Type1 subfamily. Chromosomal Location of Human Ortholog: 4q22-q24. Cellular Component: integral to plasma membrane; plasma membrane; receptor complex. Molecular Function: protein serine/threonine kinase activity; protein binding; transforming growth factor beta receptor activity, type I; metal ion binding; SMAD binding; ATP binding; transmembrane receptor protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity. Biological Process: chondrocyte development; positive regulation of bone mineralization; limb morphogenesis; protein amino acid phosphorylation; BMP signaling pathway; positive regulation of osteoblast differentiation; ovulation cycle; eye development; dorsal/ventral pattern formation; retina development in camera-type eye; proteoglycan biosynthetic process; ovarian cumulus expansion; positive regulation of cell differentiation; inflammatory response; skeletal development; retinal ganglion cell axon guidance; cartilage condensation. Disease: Chondrodysplasia, Acromesomelic, With Genital Anomalies; Brachydactyly, Type A2

48-Strip-Wells

470 USD

96-Strip-Wells

675