ELISA/assay

Canine A Disintegrin And Metalloprotease 19 ELISA Kit

MBS753120

96 Strip Wells

675 USD

ELISA Kit

Canine A Disintegrin And Metalloprotease 19 ELISA Kit

A Disintegrin And Metalloprotease 19

ADAM19 protein, partial; Disintegrin and metalloproteinase domain-containing protein 19; disintegrin and metalloproteinase domain-containing protein 19; ADAM 19; meltrin-beta; metalloprotease-disintegrin meltrin beta; metalloprotease and disintegrin dendritic antigen marker; a disintegrin and metalloproteinase domain 19 (meltrin beta); ADAM metallopeptidase domain 19; Meltrin-beta; Metalloprotease and disintegrin dendritic antigen marker; MADDAM

ADAM19

ADAM19; ADAM19; MLTNB; FKSG34; MADDAM; MLTNB; FKSG34; ADAM 19; MADDAM

ADA19_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of ADAM19. No significant cross-reactivity or interference between ADAM19 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAM19 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Intended Uses: This ADAM19 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine ADAM19. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Sensitivity: The sensitivity in this assay is 0.1 ng/mL.

Cell Biology

Principle of the assay: ADAM19 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM19 antibody and an ADAM19-HRP conjugate. The assay sample and buffer are incubated together with ADAM19-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM19 concentration since ADAM19 from samples and ADAM19-HRP conjugate compete for the anti-ADAM19 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM19 from the sample, fewer sites are left to bind ADAM19-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM19 concentration in each sample is interpolated from this standard curve.

38197238

AAH24214.2

100,848 Da

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has been demonstrated to be an active metalloproteinase, which may be involved in normal physiological processes such as cell migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. It is proposed to play a role in pathological processes, such as cancer, inflammatory diseases, renal diseases, and Alzheimer's disease. [provided by RefSeq, May 2013]

96 Strip Wells

675 USD