catalog number :
MBS752957
products type :
ELISA Kit
products full name :
Sheep Calprotectin ELISA Kit
products short name :
[Calprotectin]
other names :
[calprotectin=urinary stone protein band A {N-terminal}, partial]
products gene name :
[CP]
specificity :
This assay has high sensitivity and excellent specificity for detection of CALPRO. No significant cross-reactivity or interference between CALPRO and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALPRO and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Immunology
products description :
Intended Uses: This CALPRO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep CALPRO. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CALPRO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALPRO antibody and an CALPRO-HRP conjugate. The assay sample and buffer are incubated together with CALPRO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALPRO concentration since CALPRO from samples and CALPRO-HRP conjugate compete for the anti-CALPRO antibody binding site. Since the number of sites is limited, as more sites are occupied by CALPRO from the sample, fewer sites are left to bind CALPRO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALPRO concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB33355.1
size4 :
10x96-Strip-Wells