ELISA/assay

Human Collagen Type 5 ELISA Kit

MBS752766

48-Strip-Wells

470 USD

ELISA Kit

Human Collagen Type 5 ELISA Kit

Collagen Type 5

collagen, type V, alpha 1, isoform CRA_c; Collagen alpha-1(V) chain; collagen alpha-1(V) chain; collagen alpha-1(V) chain; collagen, type V, alpha 1

COL 5

COL5A1; COL5A1

CO5A1_HUMAN

Human

This assay has high sensitivity and excellent specificity for detection of CoL-5. No significant cross-reactivity or interference between CoL-5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CoL-5 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 ng/mL.

Cell Biology

Intended Uses: This CoL-5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CoL-5. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CoL-5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CoL-5 antibody and an CoL-5-HRP conjugate. The assay sample and buffer are incubated together with CoL-5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CoL-5 concentration since CoL-5 from samples and CoL-5-HRP conjugate compete for the anti-CoL-5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CoL-5 from the sample, fewer sites are left to bind CoL-5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CoL-5 concentration in each sample is interpolated from this standard curve.

119608538

EAW88132.1

183,560 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Focal Adhesion Pathway (83067)

This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

COL5A1: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 1 (EDS1); also known as Ehlers-Danlos syndrome gravis or severe classic type Ehlers-Danlos syndrome. EDS is a connective tissue disorder characterized by hyperextensible skin, atrophic cutaneous scars due to tissue fragility and joint hyperlaxity. EDS1 is the severe form of classic Ehlers-Danlos syndrome. Defects in COL5A1 are a cause of Ehlers-Danlos syndrome type 2 (EDS2); also known as Ehlers-Danlos syndrome mitis or mild classic type Ehlers Danlos syndrome. Belongs to the fibrillar collagen family. Protein type: Extracellular matrix; Motility/polarity/chemotaxis; Secreted, signal peptide; Cell adhesion; Secreted. Chromosomal Location of Human Ortholog: 9q34.2-q34.3. Cellular Component: extracellular matrix; collagen type V; endoplasmic reticulum lumen; extracellular region; basement membrane. Molecular Function: integrin binding; heparin binding; proteoglycan binding; extracellular matrix structural constituent; platelet-derived growth factor binding; metal ion binding. Biological Process: skin development; heart morphogenesis; blood vessel development; axon guidance; extracellular matrix organization and biogenesis; cell migration; collagen fibril organization; collagen catabolic process; extracellular matrix disassembly; integrin biosynthetic process; regulation of cellular component organization and biogenesis; fibril organization and biogenesis; cell adhesion; collagen biosynthetic process; eye morphogenesis; wound healing, spreading of epidermal cells. Disease: Ehlers-danlos Syndrome, Type I

48-Strip-Wells

470 USD

96-Strip-Wells

675