ELISA/assay

Human Ca2+/calmodulin dependent protein kinase ELISA Kit

MBS752532

48-Strip-Wells

470 USD

ELISA Kit

Human Ca2+/calmodulin dependent protein kinase ELISA Kit

Ca2+/calmodulin dependent protein kinase

Calcium/calmodulin-dependent protein kinase II, isoform M; Calcium/calmodulin-dependent protein kinase type II alpha chain; CG18069 gene product from transcript CG18069-RK; CG18069-PA; CG18069-PB; CG18069-PC; CG18069-PD; CG18069-PE; CG18069-PG; CG18069-PH; CG18069-PI; CG18069-PJ; CG18069-PK; CG18069-PL; CG18069-PM; Ca2+ calmodulin-dependent kinase II; CaM kinase; CaM kinase II; CaM-kinase-II; CaMKII-PA; CaMKII-PB; CaMKII-PC; CaMKII-PD; CaMKII-PE; CaMKII-PG; CaMKII-PH; CaMKII-PI; CaMKII-PJ; CaMKII-PK; CaMKII-PL; CaMKII-PM; Ca[2+]/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; Calcium/calmodulin-dependent protein kinase II; calcium/ calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase II; calmodulin kinase II; lethal (4) 102EFb; Calcium/calmodulin-dependent protein kinase II

CaM kinase

CaMKII; CaMKII; Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA; CaM; CG18069; CaM-kinase II alpha chain

KCC2A_DROME

Human

This assay has high sensitivity and excellent specificity for detection of CAMK. No significant cross-reactivity or interference between CAMK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CAMK and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses This CAMK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CAMK. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CAMK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CAMK antibody and an CAMK-HRP conjugate. The assay sample and buffer are incubated together with CAMK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CAMK concentration since CAMK from samples and CAMK-HRP conjugate compete for the anti-CAMK antibody binding site. Since the number of sites is limited, as more sites are occupied by CAMK from the sample, fewer sites are left to bind CAMK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CAMK concentration in each sample is interpolated from this standard curve.

442614537

NP_001259084.1

NM_001272155.1

55,482 Da

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1006020); CREB Phosphorylation Through The Activation Of CaMKII Pathway (1006026); CREB Phosphorylation Through The Activation Of Ras Pathway (1006023); Ca2+ Pathway (1005960); Cellular Response To Heat Stress Pathway (1005309); Cellular Responses To Stress Pathway (1005303); HSF1-dependent Transactivation Pathway (1005311); Neuronal System Pathway (1005991); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (1006008); Phototransduction - Fly Pathway (151779)

48-Strip-Wells

470 USD

96-Strip-Wells

675