ELISA/assay

Canine Proteoglycan 4 ELISA Kit

MBS752412

48-Strip-Wells

470 USD

ELISA Kit

Canine Proteoglycan 4 ELISA Kit

Proteoglycan 4

proteoglycan 4, partial; Proteoglycan 4; Lubricin; Megakaryocyte-stimulating factor; Superficial zone proteoglycanCleaved into the following chain:Proteoglycan 4 C-terminal part

PRG4

PRG4; MSF; SZP

PRG4_HUMAN

Canine

This assay has high sensitivity and excellent specificity for detection of PRG4. No significant cross-reactivity or interference between PRG4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRG4 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 0.1 ng/mL.

Intended Uses: This PRG4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PRG4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: PRG4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for PRG4. Standards or samples are then added to the microtiter plate wells and PRG4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PRG4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PRG4 are added to each well to "sandwich" the PRG4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain PRG4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRG4 concentration in each sample is interpolated from this standard curve.

50345548

AAT74746.1

146,468 Da

PRG4: Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface. Defects in PRG4 are the cause of camptodactyly- arthropathy-coxa vara-pericarditis syndrome (CACP); also known as Jacobs syndrome. CACP is an autosomal recessive disorder. Individuals with CACP have normal appearing joints at birth but with advancing age develop joint failure associated with noninflammatory synoviocyte hyperplasia and subintimal fibrosis of the synovial capsule. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1q25-q31. Cellular Component: extracellular space. Molecular Function: scavenger receptor activity; polysaccharide binding. Biological Process: receptor-mediated endocytosis; cell proliferation; immune response; negative regulation of interleukin-6 biosynthetic process; regulation of cell proliferation. Disease: Camptodactyly-arthropathy-coxa Vara-pericarditis Syndrome

48-Strip-Wells

470 USD

96-Strip-Wells

675