ELISA/assay

Rat Neural Cell Adhesion Molecule ELISA Kit

MBS752333

48-Strip-Wells

470 USD

ELISA Kit

Rat Neural Cell Adhesion Molecule ELISA Kit

Neural Cell Adhesion Molecule

neural cell adhesion molecule, partial; Neural cell adhesion molecule 1; neural cell adhesion molecule 1; neural cell adhesion molecule, NCAM; antigen recognized by monoclonal antibody 5.1H11; neural cell adhesion molecule 1; CD_antigen: CD56

NCAM

NCAM1; NCAM1; CD56; NCAM; MSK39; NCAM; N-CAM-1; NCAM-1

NCAM1_HUMAN

Rat

This assay has high sensitivity and excellent specificity for detection of NCAM. No significant cross-reactivity or interference between NCAM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NCAM and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Intended Uses: This NCAM ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat NCAM. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the assay: NCAM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NCAM antibody and an NCAM-HRP conjugate. The assay sample and buffer are incubated together with NCAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NCAM concentration since NCAM from samples and NCAM-HRP conjugate compete for the anti-NCAM antibody binding site. Since the number of sites is limited, as more sites are occupied by NCAM from the sample, fewer sites are left to bind NCAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NCAM concentration in each sample is interpolated from this standard curve.

241117

AAB20698.1

40,760 Da

Axon Guidance Pathway (105688); BDNF Signaling Pathway (712093); Cardiac Progenitor Differentiation Pathway (712094); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cytokine Signaling In Immune System Pathway (366171); Developmental Biology Pathway (477129); ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262); FGF Signaling Pathway (137989)

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Membrane protein, GPI anchor; Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 11q23.1. Cellular Component: Golgi membrane; cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane. Molecular Function: identical protein binding. Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion

48-Strip-Wells

470 USD

96-Strip-Wells

675