ELISA/assay

Human Integrin Alpha 6 ELISA Kit

MBS752054

48-Strip-Wells

470 USD

ELISA Kit

Human Integrin Alpha 6 ELISA Kit

Integrin Alpha 6

integrin alpha-6 isoform b

ITGAlpha 6

Human

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

Signal Transduction

Intended Uses: This IGalpha6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IGalpha6. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: IGalpha6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGalpha6 antibody and an IGalpha6-HRP conjugate. The assay sample and buffer are incubated together with IGalpha6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGalpha6 concentration since IGalpha6 from samples and IGalpha6-HRP conjugate compete for the anti-IGalpha6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGalpha6 from the sample, fewer sites are left to bind IGalpha6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGalpha6 concentration in each sample is interpolated from this standard curve.

119395742

NP_000201.2

NM_000210.2

Alpha6-Beta4 Integrin Signaling Pathway (198807); Arf6 Trafficking Events Pathway (137954); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Basigin Interactions Pathway (106065); CXCR4-mediated Signaling Events Pathway (137910); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

48-Strip-Wells

470 USD

96-Strip-Wells

675