Monkey Granulocyte Colony Stimulating Factor Receptor ELISA Kit | MyBioSource MBS751938 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Monkey Granulocyte Colony Stimulating Factor Receptor ELISA Kit

catalog :

MBS751938

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS751938

products type :

ELISA Kit

products full name :

Monkey Granulocyte Colony Stimulating Factor Receptor ELISA Kit

products short name :

Granulocyte Colony Stimulating Factor Receptor

other names :

granulocyte colony-stimulating factor receptor isoform d; Granulocyte colony-stimulating factor receptor; granulocyte colony-stimulating factor receptor; G-CSF-R; CD114 antigen; G-CSF receptor; colony stimulating factor 3 receptor (granulocyte); CD_antigen: CD114

products gene name :

GCSFR

other gene names :

CSF3R; CSF3R; CD114; GCSFR; GCSFR; G-CSF receptor; G-CSF-R

uniprot entry name :

CSF3R_HUMAN

reactivity :

Monkey

specificity :

This assay has high sensitivity and excellent specificity for detection of GCSFR. No significant cross-reactivity or interference between GCSFR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between GCSFR and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This GCSFR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey GCSFR. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: GCSFR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-GCSFR antibody and an GCSFR-HRP conjugate. The assay sample and buffer are incubated together with GCSFR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the GCSFR concentration since GCSFR from samples and GCSFR-HRP conjugate compete for the anti-GCSFR antibody binding site. Since the number of sites is limited, as more sites are occupied by GCSFR from the sample, fewer sites are left to bind GCSFR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GCSFR concentration in each sample is interpolated from this standard curve.

ncbi gi num :

27437045

ncbi acc num :

NP_758519.1

ncbi gb acc num :

NM_172313.2

ncbi mol weight :

86,609 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Pathways In Cancer (83105)

ncbi summary :

The protein encoded by this gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Alternatively spliced transcript variants have been described. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia. [provided by RefSeq, Aug 2010]

uniprot summary :

GCSFR: the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variant isoforms have been described. Protein type: Membrane protein, integral; Receptor, cytokine. Chromosomal Location of Human Ortholog: 1p35-p34.3. Cellular Component: integral to plasma membrane; extracellular region. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; receptor activity. Biological Process: neutrophil chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; signal transduction; cell adhesion. Disease: Neutrophilia, Hereditary

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage