product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Eukaryotic Translation Initiation Factor 2A ELISA Kit
catalog :
MBS751823
quantity :
48-Strip-Wells
price :
470 USD
more info or order :
product information
catalog number :
MBS751823
products type :
ELISA Kit
products full name :
Rat Eukaryotic Translation Initiation Factor 2A ELISA Kit
products short name :
Eukaryotic Translation Initiation Factor 2A
other names :
eukaryotic translation initiation factor 2A; Eukaryotic translation initiation factor 2A; eukaryotic translation initiation factor 2A; 65 kDa eukaryotic translation initiation factor 2A; eukaryotic translation initiation factor 2A, 65kDa; 65 kDa eukaryotic translation initiation factor 2ACleaved into the following chain:Eukaryotic translation initiation factor 2A, N-terminally processed
products gene name :
EIF2A
other gene names :
EIF2A; EIF2A; CDA02; EIF-2A; MST089; MSTP004; MSTP089; CDA02; MSTP004; MSTP089; eIF-2A
uniprot entry name :
EIF2A_HUMAN
reactivity :
Rat
specificity :
This assay has high sensitivity and excellent specificity for detection of EIF-2A. No significant cross-reactivity or interference between EIF-2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between EIF-2A and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0 pg/mL.
other info2 :
Intended Uses: This EIF-2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat EIF-2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
. Principle of the assay: EIF-2A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for EIF-2A. Standards or samples are then added to the microtiter plate wells and EIF-2A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of EIF-2A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for EIF-2A are added to each well to "sandwich" the EIF-2A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain EIF-2A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The EIF-2A concentration in each sample is interpolated from this standard curve.
ncbi gi num :
54873624
ncbi acc num :
NP_114414.2
ncbi gb acc num :
NM_032025.3
ncbi mol weight :
41,339 Da
ncbi pathways :
IL-6 Signaling Pathway (198864); TGF-beta Receptor Signaling Pathway (138024)
ncbi summary :
EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002 [PubMed 12133843]).[supplied by OMIM, Mar 2008]
uniprot summary :
EIF2A: Functions in the early steps of protein synthesis of a small number of specific mRNAs. Acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. In contrast to the eIF- 2 complex, it binds methionyl-tRNAi to 40 S subunits in a codon- dependent manner, whereas the eIF-2 complex binds methionyl-tRNAi to 40 S subunits in a GTP-dependent manner. May act by impiging the expression of specific proteins. Belongs to the WD repeat EIF2A family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Translation. Chromosomal Location of Human Ortholog: 3q25.1. Cellular Component: extracellular space; cytoplasm; eukaryotic translation initiation factor 2 complex. Molecular Function: protein binding; translation initiation factor activity; ribosome binding; tRNA binding. Biological Process: regulation of translation; positive regulation of signal transduction; ribosome assembly; translational initiation; SREBP-mediated signaling pathway; protein amino acid phosphorylation
size1 :
48-Strip-Wells
price1 :
470 USD
size2 :
96-Strip-Wells
price2 :
675
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
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