Human Costimulatory molecules B7H4 ELISA Kit | MyBioSource MBS751659 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Costimulatory molecules B7H4 ELISA Kit

catalog :

MBS751659

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS751659

products type :

ELISA Kit

products full name :

Human Costimulatory molecules B7H4 ELISA Kit

products short name :

Costimulatory molecules B7H4

other names :

B7-H4; V-set domain-containing T-cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; V-set domain containing T cell activation inhibitor 1; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x

products gene name :

B7 H4

other gene names :

VTCN1; VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; B7H4; UNQ659/PRO1291

uniprot entry name :

VTCN1_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of B7H4. No significant cross-reactivity or interference between B7H4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between B7H4 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This B7H4 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human B7H4. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Priciple of the assay: B7H4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-B7H4 antibody and an B7H4-HRP conjugate. The assay sample and buffer are incubated together with B7H4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the B7H4 concentration since B7H4 from samples and B7H4-HRP conjugate compete for the anti-B7H4 antibody binding site. Since the number of sites is limited, as more sites are occupied by B7H4 from the sample, fewer sites are left to bind B7H4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The B7H4 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

70997756

ncbi acc num :

AAZ17406.1

ncbi mol weight :

20,743 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

ncbi summary :

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

VTCN1: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor- associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. Belongs to the immunoglobulin superfamily. BTN/MOG family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 1p13.1. Cellular Component: integral to membrane; external side of plasma membrane. Molecular Function: receptor binding. Biological Process: response to protozoan; immune system process; positive regulation of T cell proliferation; negative regulation of T cell activation

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage