Mouse Mast Cell Chymase ELISA Kit | MyBioSource MBS751568 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Mast Cell Chymase ELISA Kit

catalog :

MBS751568

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS751568

products type :

ELISA Kit

products full name :

Mouse Mast Cell Chymase ELISA Kit

products short name :

Mast Cell Chymase

other names :

mast cell chymase; Chymase; chymase; alpha-chymase; chymase, heart; chymase, mast cell; mast cell protease I; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; chymase 1, mast cell; Alpha-chymase; Mast cell protease I

products gene name :

MCC

other gene names :

CMA1; CMA1; CYH; MCT1; chymase; CYH; CYM

uniprot entry name :

CMA1_HUMAN

reactivity :

Mouse

specificity :

This assay has high sensitivity and excellent specificity for detection of MC. No significant cross-reactivity or interference between MC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MC and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

other info2 :

Intended Uses: This MC ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MC. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products description :

Principle of the assay: MC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MC antibody and an MC-HRP conjugate. The assay sample and buffer are incubated together with MC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MC concentration since MC from samples and MC-HRP conjugate compete for the anti-MC antibody binding site. Since the number of sites is limited, as more sites are occupied by MC from the sample, fewer sites are left to bind MC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MC concentration in each sample is interpolated from this standard curve.

ncbi gi num :

180542

ncbi acc num :

AAA52020.1

ncbi mol weight :

27,325 Da

ncbi pathways :

ACE Inhibitor Pathway (198763); Activation Of Matrix Metalloproteinases Pathway (576264); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Metabolism Of Angiotensinogen To Angiotensins Pathway (685555); Metabolism Of Proteins Pathway (106230); Peptide Hormone Metabolism Pathway (771603); Renin-angiotensin System Pathway (83075); Renin-angiotensin System Pathway (486); Signal Transduction Pathway (477114)

ncbi summary :

This gene product is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Angiotensin II has been implicated in blood pressure control and in the pathogenesis of hypertension, cardiac hypertrophy, and heart failure. Thus, this gene product is a target for cardiovascular disease therapies. This gene maps to 14q11.2 in a cluster of genes encoding other proteases. [provided by RefSeq, Jul 2008]

uniprot summary :

CMA1: Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. Belongs to the peptidase S1 family. Granzyme subfamily. Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.21.39. Chromosomal Location of Human Ortholog: 14q11.2. Cellular Component: extracellular matrix; extracellular space; extracellular region; intracellular. Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity; peptide binding. Biological Process: extracellular matrix disassembly; interleukin-1 beta biosynthetic process; extracellular matrix organization and biogenesis; positive regulation of angiogenesis; cellular protein metabolic process; midbrain development; regulation of inflammatory response; protein processing; angiotensin maturation; proteolysis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage