Canine Syndecan-1/CD138 ELISA Kit | MyBioSource MBS751407 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Syndecan-1/CD138 ELISA Kit

catalog :

MBS751407

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS751407

products type :

ELISA Kit

products full name :

Canine Syndecan-1/CD138 ELISA Kit

products short name :

Syndecan-1/CD138

other names :

syndecan-1; Syndecan-1; syndecan-1; CD138 antigen; syndecan proteoglycan 1; heparan sulfate proteoglycan fibroblast growth factor receptor; syndecan 1; CD_antigen: CD138

products gene name :

SDC1/CD138

other gene names :

SDC1; SDC1; SDC; CD138; SYND1; syndecan; SDC; SYND1

uniprot entry name :

SDC1_HUMAN

reactivity :

Canine

specificity :

This assay has high sensitivity and excellent specificity for detection of SDC-1. No significant cross-reactivity or interference between SDC-1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SDC-1 and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Sandwich. Sensitivity: 1.0pg/mL.

products categories :

Cell Biology

products description :

Intended Uses: This SDC-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine SDC-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay SDC-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDC-1. Standards or samples are then added to the microtiter plate wells and SDC-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDC-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SDC-1 are added to each well to "sandwich" the SDC-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDC-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDC-1 concentration in each sample is interpolated from this standard curve.

ncbi gi num :

55749480

ncbi acc num :

NP_001006947.1

ncbi gb acc num :

NM_001006946.1

ncbi mol weight :

32,462 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Arf6 Trafficking Events Pathway (137954); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Chylomicron-mediated Lipid Transport Pathway (106157); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479)

ncbi summary :

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

uniprot summary :

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family. Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Extracellular matrix. Chromosomal Location of Human Ortholog: 2p24.1. Cellular Component: lysosomal lumen; cell surface; focal adhesion; protein complex; integral to plasma membrane; Golgi lumen; cytoplasm; plasma membrane; external side of plasma membrane. Molecular Function: protein C-terminus binding; protein binding; glycoprotein binding. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; myoblast development; response to cAMP; wound healing; glycosaminoglycan metabolic process; striated muscle cell development; response to toxin; response to glucocorticoid stimulus; lipoprotein metabolic process; pathogenesis; Wnt receptor signaling pathway through beta-catenin; Sertoli cell development; odontogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; response to hydrogen peroxide; ureteric bud development; carbohydrate metabolic process; inflammatory response; response to calcium ion; retinoid metabolic process

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage