catalog number :
MBS751320
products type :
ELISA Kit
products full name :
Rabbit Immunoglobulin G1 ELISA Kit
products short name :
Immunoglobulin G1
other names :
immunoglobulin G1 Fc region, partial
products gene name :
IgG1
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive
other info2 :
Intended Uses: This IgG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IgG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications. !Intended Uses: This IgG1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit IgG1. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products categories :
Immunology
products description :
Principle of the Assay: IgG1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IgG1 antibody and an IgG1-HRP conjugate. The assay sample and buffer are incubated together with IgG1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgG1 concentration since IgG1 from samples and IgG1-HRP conjugate compete for the anti-IgG1 antibody binding site. Since the number of sites is limited, as more sites are occupied by IgG1 from the sample, fewer sites are left to bind IgG1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG1 concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AFF59210.1