Rat Amylase Alpha 2, Pancreatic ELISA Kit | MyBioSource MBS750781 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Amylase Alpha 2, Pancreatic ELISA Kit

catalog :

MBS750781

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS750781

products type :

ELISA Kit

products full name :

Rat Amylase Alpha 2, Pancreatic ELISA Kit

products short name :

Amylase Alpha 2, Pancreatic

other names :

amylase; Pancreatic alpha-amylase; pancreatic alpha-amylase; glycogenase; found in the pancreas; pancreatic amylase 2A; pancreatic amylase alpha 2A; amylase, pancreatic, alpha-2A; 1,4-alpha-D-glucan glucanohydrolase; amylase, alpha 2A (pancreatic); 1,4-alpha-D-glucan glucanohydrolase

products gene name :

AMY2

other gene names :

AMY2A; AMY2A; PA; AMY2; AMY2B; PA

uniprot entry name :

AMYP_HUMAN

reactivity :

Rat

specificity :

This assay has high sensitivity and excellent specificity for detection of AMYalpha. No significant cross-reactivity or interference between AMYalpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AMYalpha and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 0.1 ng/mL.

products description :

Intended Uses: This AMYalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat AMYalpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay AMYalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AMYalpha antibody and an AMYalpha-HRP conjugate. The assay sample and buffer are incubated together with AMYalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMYalpha concentration since AMYalpha from samples and AMYalpha-HRP conjugate compete for the anti-AMYalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by AMYalpha from the sample, fewer sites are left to bind AMYalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMYalpha concentration in each sample is interpolated from this standard curve.

ncbi gi num :

529397

ncbi acc num :

AAA51724.1

ncbi mol weight :

30,302 Da

ncbi pathways :

Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Digestion Of Dietary Carbohydrate Pathway (106197); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196); Myoclonic Epilepsy Of Lafora Pathway (980469); Pancreatic Secretion Pathway (169306)

ncbi summary :

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]

uniprot summary :

AMY2A: Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the pancreas. [provided by RefSeq, Jul 2008]. Protein type: EC 3.2.1.1; Secreted; Secreted, signal peptide; Hydrolase; Carbohydrate Metabolism - starch and sucrose. Chromosomal Location of Human Ortholog: 1p21. Cellular Component: extracellular space; extracellular region. Molecular Function: alpha-amylase activity; calcium ion binding; chloride ion binding. Biological Process: polysaccharide digestion; carbohydrate catabolic process; carbohydrate metabolic process; pathogenesis

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage