catalog number :
MBS750306
products type :
ELISA Kit
products full name :
Sheep Pregnancy-Associated Glycoproteins ELISA Kit
products short name :
Pregnancy-Associated Glycoproteins
products gene name :
PAGs
specificity :
This assay has high sensitivity and excellent specificity for detection of PAG. No significant cross-reactivity or interference between PAG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PAG and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
other info1 :
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 ng/mL.
other info2 :
Intended Uses: This PAG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep PAG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
products description :
Principle of the assay: PAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PAG antibody and an PAG-HRP conjugate. The assay sample and buffer are incubated together with PAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PAG concentration since PAG from samples and PAG-HRP conjugate compete for the anti-PAG antibody binding site. Since the number of sites is limited, as more sites are occupied by PAG from the sample, fewer sites are left to bind PAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PAG concentration in each sample is interpolated from this standard curve.