ELISA/assay

Mouse Interleukin 36 ELISA Kit

MBS750241

48-Strip-Wells

470 USD

ELISA Kit

Mouse Interleukin 36 ELISA Kit

Interleukin 36

interleukin-36 alpha; Interleukin-36 alpha; interleukin-36 alpha; FIL1 epsilon; IL-1 epsilon; interleukin-1 epsilon; IL-1F6 (FIL-1-epsilon); interleukin 1, epsilon; interleukin-1 family member 6; interleukin 1 family, member 6 (epsilon); interleukin 36, alpha; FIL1 epsilon; Interleukin-1 epsilon; IL-1 epsilon; Interleukin-1 family member 6; IL-1F6

IL-36

IL36A; IL36A; FIL1; FIL1E; IL1F6; IL-1F6; IL1(EPSILON); FIL1(EPSILON); FIL1E; IL1E; IL1F6; IL-1 epsilon; IL-1F6

IL36A_HUMAN

Mouse

This assay has high sensitivity and excellent specificity for detection of IL-36. No significant cross-reactivity or interference between IL-36 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-36 and all the analogues, therefore, cross reaction may still exist in some cases.

Store all reagents at 2-8 degree C.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

Intended Uses: This IL-36 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse IL-36. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Immunology

Principle of the assay: IL-36 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-36 antibody and an IL-36-HRP conjugate. The assay sample and buffer are incubated together with IL-36-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-36 concentration since IL-36 from samples and IL-36-HRP conjugate compete for the anti-IL-36 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-36 from the sample, fewer sites are left to bind IL-36-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-36 concentration in each sample is interpolated from this standard curve.

7657092

NP_055255.1

NM_014440.1

17,684 Da

IL1F6: Belongs to the IL-1 family. Protein type: Cytokine. Chromosomal Location of Human Ortholog: 2q12-q14.1. Cellular Component: extracellular space; extracellular region. Molecular Function: interleukin-1 receptor binding; cytokine activity. Biological Process: cytokine and chemokine mediated signaling pathway; innate immune response; positive regulation of interleukin-6 production; immune response; inflammatory response

48-Strip-Wells

470 USD

96-Strip-Wells

675