Human Interleukin 32 Alpha ELISA Kit | MyBioSource MBS750027 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interleukin 32 Alpha ELISA Kit

catalog :

MBS750027

quantity :

48-Strip-Wells

price :

470 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
El Far M, Kouassi P, Sylla M, Zhang Y, Fouda A, Fabre T, et al. Proinflammatory isoforms of IL-32 as novel and robust biomarkers for control failure in HIV-infected slow progressors. Sci Rep. 2016;6:22902 pubmed publisher

product information

catalog number :

MBS750027

products type :

ELISA Kit

products full name :

Human Interleukin 32 Alpha ELISA Kit

products short name :

Interleukin 32 Alpha

other names :

interleukin-32 isoform A; Interleukin-32; interleukin-32; interleukin-32 eta; interleukin-32 small; interleukin-32 theta; natural killer cells protein 4; natural killer cell transcript 4; tumor necrosis factor alpha-inducing factor; interleukin 32; Natural killer cells protein 4; Tumor necrosis factor alpha-inducing factor

products gene name :

IL 32a

other gene names :

IL32; IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma; NK4; TAIF; IL-32

uniprot entry name :

IL32_HUMAN

reactivity :

Human

specificity :

This assay has high sensitivity and excellent specificity for detection of IL-32alpha. No significant cross-reactivity or interference between IL-32alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-32alpha and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

other info1 :

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate. Assay Type: Competitive. Sensitivity: 1.0 pg/mL.

other info2 :

Intended Uses: This IL-32alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IL-32alpha. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

products categories :

Immunology

products description :

Principle of the assay: IL-32alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-32alpha antibody and an IL-32alpha-HRP conjugate. The assay sample and buffer are incubated together with IL-32alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-32alpha concentration since IL-32alpha from samples and IL-32alpha-HRP conjugate compete for the anti-IL-32alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-32alpha from the sample, fewer sites are left to bind IL-32alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-32alpha concentration in each sample is interpolated from this standard curve.

ncbi gi num :

61658634

ncbi acc num :

NP_001012651.1

ncbi gb acc num :

NM_001012633.1

ncbi mol weight :

17,003 Da

ncbi summary :

This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

uniprot summary :

IL32: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF- alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Cell adhesion. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular space; membrane. Molecular Function: protein binding; cytokine activity. Biological Process: immune response; defense response; cell adhesion

size1 :

48-Strip-Wells

price1 :

470 USD

size2 :

96-Strip-Wells

price2 :

675

more info or order :

MyBioSource product webpage