catalog number :
MBS749839
products type :
ELISA Kit
products full name :
Bovine Vitamin B2 ELISA Kit
products short name :
[Vitamin B2]
products gene name :
[VB2]
specificity :
This assay has high sensitivity and Limited by current skills and knowledge, this kit might not able to differentiate VB12 from its analogues.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 1.0 pg/mL.
products description :
Intended Uses: This VB12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human VB12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: VB12 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-VB12 antibody and an VB12-HRP conjugate. The assay sample and buffer are incubated together with VB12-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VB12 concentration since VB12 from samples and VB12-HRP conjugate compete for the anti-VB12 antibody binding site. Since the number of sites is limited, as more sites are occupied by VB12 from the sample, fewer sites are left to bind VB12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VB12 concentration in each sample is interpolated from this standard curve.
size4 :
10x96-Strip-Wells