catalog number :
MBS749762
products type :
ELISA Kit
products full name :
Porcine Calprotectin ELISA Kit
products short name :
[Calprotectin]
other names :
[calprotectin=urinary stone protein band A {N-terminal}, partial]
products gene name :
[CP]
specificity :
This assay has high sensitivity and excellent specificity for detection of CALCRL. No significant cross-reactivity or interference between CALCRL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CALCRL and all the analogues, therefore, cross reaction may still exist in some cases.
storage stability :
Store all reagents at 2-8 degree C.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ng/mL.
products categories :
Immunology
products description :
Intended Uses: This CALCRL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CALCRL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: CALCRL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CALCRL antibody and an CALCRL-HRP conjugate. The assay sample and buffer are incubated together with CALCRL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALCRL concentration since CALCRL from samples and CALCRL-HRP conjugate compete for the anti-CALCRL antibody binding site. Since the number of sites is limited, as more sites are occupied by CALCRL from the sample, fewer sites are left to bind CALCRL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALCRL concentration in each sample is interpolated from this standard curve.
ncbi acc num :
AAB33355.1
size4 :
10x96-Strip-Wells