Canine Fibroblast Growth Factor 19 ELISA Kit | MyBioSource MBS749333 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Canine Fibroblast Growth Factor 19 ELISA Kit

catalog :

MBS749333

quantity :

48-Strip-Wells

price :

440 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS749333

products type :

ELISA Kit

products full name :

Canine Fibroblast Growth Factor 19 ELISA Kit

products short name :

[Fibroblast Growth Factor 19]

other names :

[fibroblast growth factor 19; Fibroblast growth factor 19; fibroblast growth factor 19; FGF-19; fibroblast growth factor 19]

products gene name :

[FGF19]

other gene names :

[FGF19; FGF19; UNQ334/PRO533; FGF-19]

uniprot entry name :

FGF19_HUMAN

reactivity :

Canine

specificity :

This assay has high sensitivity and excellent specificity for detection of FFA. No significant cross-reactivity or interference between FFA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FFA and all the analogues, therefore, cross reaction may still exist in some cases.

storage stability :

Store all reagents at 2-8 degree C.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate. Assay Type: Quantitative Competitive. Sensitivity: 0.1 ug/mL.

products categories :

Signal Transduction

products description :

Intended Uses: This FFA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat FFA. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Principle of the Assay: FFA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FFA antibody and an FFA-HRP conjugate. The assay sample and buffer are incubated together with FFA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FFA concentration since FFA from samples and FFA-HRP conjugate compete for the anti-FFA antibody binding site. Since the number of sites is limited, as more sites are occupied by FFA from the sample, fewer sites are left to bind FFA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FFA concentration in each sample is interpolated from this standard curve.

ncbi gi num :

4826726

ncbi acc num :

NP_005108.1

ncbi gb acc num :

NM_005117.2

ncbi mol weight :

24,003 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); FGF Signaling Pathway (137989); FGFR Ligand Binding And Activation Pathway (106344)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. This growth factor is a high affinity, heparin dependent ligand for FGFR4. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. [provided by RefSeq, Jul 2008]

uniprot summary :

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family. Protein type: Cytokine; Secreted; Cell development/differentiation; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q13.1. Cellular Component: extracellular region. Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of glucose import; nervous system development; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; heart development; positive regulation of cell proliferation; insulin receptor signaling pathway; innate immune response; positive regulation of JNK cascade; positive regulation of protein amino acid phosphorylation; neural crest cell migration

size1 :

48-Strip-Wells

price1 :

440 USD

size2 :

96-Strip-Wells

price2 :

640

size3 :

5x96-Strip-Wells

price3 :

2895

size4 :

10x96-Strip-Wells

price4 :

5415

more info or order :

MyBioSource product webpage